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). In the free ChVLig-AMP, the OB area is reflected faraway from the NTase domain to totally expose the DNA-binding surface area higher than the AMP-binding pocket. The peptide section that's destined to become the latch is disordered inside the free ligase and sensitive to proteolysis, but this section is shielded from proteolysis when ChVLig binds to nicked DNA.
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